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Recombinant human ITLN1 protein was expressed in E.coli and purified by using conventional chromatography techniques (17-313 aa).ITLN1, also known as intelectin1 or omentin, has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake
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For quantitative detection of human Omentin intelectin 1 in cell culture supernates serum and plasma heparin EDTA citrate
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Image Search Results
Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Article Title: hESC-Derived Epicardial Cells Promote Repair of Infarcted Hearts in Mouse and Swine.
doi: 10.1002/advs.202300470
Figure Lengend Snippet: Figure 5. hEPs abundantly secrete ITLN1 and inhibit IFN-I responses in macrophages. A) Western blotting analysis of IFN-I signaling-related proteins in the LV tissues at day 1 post-MI. n = 4. B) Mass spectrometry (MS) analysis revealed top eight enriched proteins in the co-immunoprecipitated (co-IP) precipitate of IFN-𝛽and the proteins in the hEP-conditioned medium (hEP-CdM). C) qRT-PCR analysis of the transcriptional levels of candidates screened by MS in the hESC-derived cardiomyocytes (hCMs) and hEPs. n = 3. D) ELISA analysis of the hITLN1 level in the CdM of hEPs and hCMs. n = 3 to 4. E) ELISA analysis of the hITLN1 protein level in the LV tissues at day 1 post-MI. n = 3 to 4. F) Representative image of co-immunofluorescent staining for hMito and ITLN1 at day 3 post-MI in the infarcted hearts treated with hEPs. Scale bar: 50 μm. G) Co-IP analysis showed the interaction between ITLN1 and hFc-tagged IFN𝛽(hFc-IFN𝛽) in the hEP-CdM. n = 3. H) Representative images showing the morphology of mouse peritoneal macrophages after 24 h with various treatments. n = 3. Serum-free DMEM was used as a vehicle control. The cells were treated with vehicle supplemented with 300 ng mL−1
Article Snippet: ELISA: The concentration of hITLN1 was measured using a
Techniques: Western Blot, Mass Spectrometry, Immunoprecipitation, Co-Immunoprecipitation Assay, Quantitative RT-PCR, Derivative Assay, Enzyme-linked Immunosorbent Assay, Staining, Control